Comparison of several indium-111 ligands in labeling blood cells: effect of diethylpyrocarbonate and CO/sub 2/
The effect of oxine sulfate, oxine sulfonate, tropolone, and Merc (2 mercaptopyridine-1-oxide) were compared with oxine, with respect to their capability of labeling blood cells when complexed to indium-111 (/sup 111/In). Indium-111 oxine sulfate performed similarly to (/sup 111/In)oxine with regard to cell labeling capability. Indium-111 oxine sulfonate had no labeling ability. Indium-111 tropolone and Merc were not superior to (/sup 111/In)oxine as cell labeling agents. Carbon dioxide (CO/sub 2/) and a CO/sub 2/ generating compound, diethyl pyrocarbonate, dramatically improved the cell labeling ability in plasma of (/sup 111/In)tropolone and Merc. In the case of oxine, this improvement was less distinct. Theoretical aspects of the CO/sub 2/ cell labeling stimulating effect are discussed in terms of intra- and extracellular transferrin and lactoferrin iron (indium) binding capacity. Indium-111 tropolone behaved favorably with respect to inhibition of leukocyte migration, compared with oxine and Merc. Combined with the property of easy cell labeling and good solubility in water, also in the complexed state, tropolone must be regarded as the most suitable cell labeling ligand.
- Research Organization:
- B.V. Research and Development Department, Petten, The Netherlands
- OSTI ID:
- 6274874
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 6; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Comparison of In-111-MERC leukocytes with In-111-Oxine leukocytes for abscess detection
No difference in sensitivity for occult infection between tropolone- and oxine-labeled indium-111 leukocytes
Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBONATES
CATTLE
CHALCOGENIDES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LEUKOCYTES
LIGANDS
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOMETALLIC COMPOUNDS
OXIDES
OXINE
OXYGEN COMPOUNDS
PYRIDINES
QUINOLINES
RADIOISOTOPES
RUMINANTS
VERTEBRATES