Effects of irradiation on canine tracheal epithelium: a physiological and morphological correlate

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148709064302· OSTI ID:6273103

Man, S F; Logus, J W; Mok, K; Yamamoto, H; Ahmed, I H; Man, G C; Hulbert, W C

We delivered 20 Gy irradiation in one fraction to a 6 cm segment of trachea in 11 dogs. Tracheal mucous transport was studied before and whenever possible at weekly intervals after irradiation using a gamma camera system and /sup 99m/technetium labeled sulfur colloid. Ten of the eleven animals were sacrificed at three different time intervals (1-2, 15-16 and 30-34 weeks) post-irradiation, and the tracheal epithelium removed for studies using Ussing chambers followed by preparation for microscopic analysis. Mucous transport along the length of the trachea was normal before irradiation, but following irradiation it became abnormal in the irradiated zone. Compared to the epithelium from the cranial and caudal segments, the irradiated epithelium had similar bioelectric measurements (potential difference, short-circuit current and resistance) and mannitol permeability. Also, the changes in the bioelectric measurements following indomethacin (10(-6) M) and epinephrine (10(-6) M) used sequentially, were similar in both the control and irradiated tissues. Scanning electron microscopic analysis of the irradiated zone revealed patches of nonciliated epithelial cells among the ciliates. We conclude that irradiation caused a persistent replacement of ciliated cells with nonciliates throughout the entire study period and that this alteration impaired mucous transport but did not affect epithelial ion secretion or barrier function.

• Research Organization: Univ. of Alberta, Edmonton
• OSTI ID: 6273103
• Journal Information: Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 3; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
COLLOIDS
DISPERSIONS
DOGS
ELECTRON MICROSCOPY
ELECTROPHYSIOLOGY
EPITHELIUM
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IRRADIATION
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
PERMEABILITY
PHYSIOLOGY
RADIATION EFFECTS
RADIOISOTOPES
RESPIRATORY SYSTEM
SCANNING ELECTRON MICROSCOPY
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUES
TRACHEA
VERTEBRATES
YEARS LIVING RADIOISOTOPES