Effects of SO/sub 2/ exposure on canine pulmonary epithelial functions

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148909087852· OSTI ID:6066650

Man, S F; Hulbert, W C; Man, G; Mok, K; Williams, D J

We examined the effects of a single exposure of high concentrations of sulfur dioxide (SO/sub 2/) on the pulmonary epithelium in adult dogs over a period of several weeks. Mucociliary tracheal transport rates and alveolar clearance of /sup 99m/Tc-labeled diethylene triamine pentacetate (/sup 99m/TcO/sub 4/minus//) were measured in vivo, before and immediately after inhalation of 100 ppm or 500 ppm SO/sub 2/, and then weekly for 3-5 weeks. At the completion of the in vivo studies, tracheal epithelium was studied in Ussing chambers for bioelectric properties (short-circuited current, transepithelial potential difference), nonelectrolyte permeability for calculation of pore sizes, and changes in bioelectric properties following pharmacological manipulations. These tissues were then fixed for scanning electron microscopy studies. Additional dogs were sacrificed for microscopy studies at several time intervals to provide a histological basis for the altered mucociliary transport. We found that despite marked derangement of mucociliary transport caused by damage to the ciliated cells, recovery occurred over a period of several weeks, and alveolar permeability as assessed by the radioaerosol technique did not change. We concluded that the solubility of SO/sub 2/ and perhaps a more severe damaging effect of SO/sub 2/ specific on the ciliated cells might be the explanation for the observations.

• Research Organization: Univ. of Alberta, Edmonton (Canada)
• OSTI ID: 6066650
• Journal Information: Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 15:2; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
AEROSOLS
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHALCOGENIDES
CHELATING AGENTS
CLEARANCE
COLLOIDS
DISPERSIONS
DOGS
DRUGS
DTPA
ELECTRON MICROSCOPY
EPITHELIUM
EXCRETION
HOURS LIVING RADIOISOTOPES
INHALATION
INTAKE
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUNG CLEARANCE
LUNGS
MAMMALS
METABOLISM
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
SCANNING ELECTRON MICROSCOPY
SENSITIVITY
SOLS
SULFUR COMPOUNDS
SULFUR DIOXIDE
SULFUR OXIDES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUES
TOXICITY
TRACER TECHNIQUES
TRACHEA
VERTEBRATES
YEARS LIVING RADIOISOTOPES