Ciliated cells in vitamin A-deprived cultured hamster tracheal epithelium do divide

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · In Vitro Cell. Develop. Biol.; (United States)

DOI:https://doi.org/10.1007/BF02623905· OSTI ID:6559082

Rutten, A A; Beems, R B; Wilmer, J W; Feron, V J

The pseudostratified tracheal epithelium, composed of a heterogeneous phenotypically varying cell population, was studied with respect to the in vitro cell proliferative activity of differentiated epithelial cells. Ciliated tracheal epithelial cells so far have been considered to be terminally differentiated, nonproliferating cells. Tracheal organ cultures obtained from vitamin A-deprived Syrian Golden hamsters were cultured in a vitamin A-deficient, serum-free, hormone-supplemented medium. In vitamin A-deprived tracheal epithelium treated with physiologically active all-trans retinol and low cigarette-smoke condensate concentrations it is possible to stimulate the cell proliferation of both basal and columnar cells. Therefore, the probability of finding proliferating columnar cells was increased compared with the in vivo and the vitamin A-deprived situation in which cell proliferative activity is relatively low. In the presence of cigarette-smoke condensate in a noncytotoxic concentration, basal, small mucous granule, ciliated, and indifferent tracheal epithelial cells incorporated (methyl-3H)-thymidine into the DNA during the S phase. The finding that ciliated cells were labeled was supported by serial sections showing the same labeled ciliated cell in two section planes separated by 2 to 3 micron, without labeled epithelial cells next to the ciliated cell. Furthermore, a ciliated tracheal epithelial cell incorporating (methyl-/sup 3/H)thymidine into DNA was also seen in tracheal cultures of vitamin A-deprived hamsters treated with all-trans retinol in a physiologic concentration.

• Research Organization: TNO-CIVO Toxicology and Nutrition Institute, Zeist (Netherlands)
• OSTI ID: 6559082
• Journal Information: In Vitro Cell. Develop. Biol.; (United States), Journal Name: In Vitro Cell. Develop. Biol.; (United States) Vol. 24:9; ISSN ICDBE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
BODY
CARBOXYLIC ACID ESTERS
CELL DIFFERENTIATION
CELL PROLIFERATION
DNA
EPITHELIUM
ESTERS
HAMSTERS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RESPIRATORY SYSTEM
RETINOIC ACID
RIBOSIDES
RODENTS
THYMIDINE
TISSUES
TOBACCO
TRACHEA
TRITIUM COMPOUNDS
VERTEBRATES
VITAMIN A
VITAMINS