Tier II mutagenic screening of 13 NIOSH priority compounds: individual compound report, n,n-dimethylformamide

Technical Report · Fri May 01 04:00:00 EDT 1981

OSTI ID:6271327

McGregor, D B

N,N-Dimethylformamide (68122) (NND) was subjected to a tier II mutagenic test screening program. The assays included unscheduled DNA synthesis (UDS) assay in human diploid fibroblasts with exposure of 3 hours duration and concentrations up to 9,220 micrograms per milliliter of culture medium; dominant lethal test in male rats with exposure to atmospheres containing 10 parts per million (ppm) or 400ppm NND; sperm abnormality test in male mice using the same exposure conditions; cytogenetic test in male and female rat bone marrow cells using the same exposure conditions or a single exposure of 7 hours duration; and sex linked recessive lethal test in Drosphila-melanogaster with exposure to an atmosphere of 400ppm for 2.25 hours. There was no increase in UDS in cells treated with NND nor were there effects attributable to NND in the dominant lethal test on pregnancy frequency, numbers of corpora lutea or implantations, or the frequency of early deaths. Abnormal sperm frequency was not affected, the frequencies of chromosomal aberrations were not increased in rat bone marrow cells, and sex linked recessive lethal frequency was not increased in Drosphila. The author concludes that NND was devoid of genetic effects.

• Research Organization: Inveresk Research International Ltd., Musselburgh (UK)
• OSTI ID: 6271327
• Report Number(s): PB-83-133900
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
DNA
FORMAMIDE
GENETIC EFFECTS
LABORATORY ANIMALS
MAMMALS
MUTAGEN SCREENING
NUCLEIC ACIDS
OPTIMIZATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RATS
RODENTS
SCREENING
SYNTHESIS
VERTEBRATES