NMR imaging and spectroscopy of the mammalian central nervous system after heavy ion radiation

Technical Report · Sat Sep 01 04:00:00 EDT 1984

DOI:https://doi.org/10.2172/6268623· OSTI ID:6268623

Richards, T

NMR imaging, NMR spectroscopic, and histopathologic techniques were used to study the proton relaxation time and related biochemical changes in the central nervous system after helium beam in vivo irradiation of the rodent brain. The spectroscopic observations reported in this dissertation were made possible by development of methods for measuring the NMR parameters of the rodent brain in vivo and in vitro. The methods include (1) depth selective spectroscopy using an optimization of rf pulse energy based on a priori knowledge of N-acetyl aspartate and lipid spectra of the normal brain, (2) phase-encoded proton spectroscopy of the living rodent using a surface coil, and (3) dual aqueous and organic tissue extraction technique for spectroscopy. Radiation induced increases were observed in lipid and p-choline peaks of the proton spectrum, in vivo. Proton NMR spectroscopy measurements on brain extracts (aqueous and organic solvents) were made to observe chemical changes that could not be seen in vivo. Radiation-induced changes were observed in lactate, GABA, glutamate, and p-choline peak areas of the aqueous fraction spectra. In the organic fraction, decreases were observed in peak area ratios of the terminal-methyl peaks, the N-methyl groups of choline, and at a peak at 2.84 ppM (phosphatidyl ethanolamine and phosphatidyl serine resonances) relative to TMS. With histology and Evans blue injections, blood-brain barrier alternations were seen as early as 4 days after irradiation. 83 references, 53 figures.

• Research Organization: Lawrence Berkeley Lab., CA (USA)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6268623
• Report Number(s): LBL-18148; ON: DE85004509
• Country of Publication: United States
• Language: English

Related Subjects

560114 -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
B CODES
BEAMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
COMPUTER CODES
D CODES
DATA
ELEMENTS
EXPERIMENTAL DATA
EXTRACTION
FLUIDS
FOURIER TRANSFORMATION
GASES
HELIUM
HISTOLOGY
IN VIVO
INFORMATION
INTEGRAL TRANSFORMATIONS
ION BEAMS
MAGNETIC RESONANCE
MAMMALS
N CODES
NERVOUS SYSTEM
NONMETALS
NUCLEAR MAGNETIC RESONANCE
NUMERICAL DATA
ORGANS
RADIATION EFFECTS
RARE GASES
RATS
RESONANCE
RODENTS
SEPARATION PROCESSES
SOLVENT EXTRACTION
SPECTROSCOPY
TRANSFORMATIONS
U CODES
VERTEBRATES