NMR imaging and spectroscopy of the mammalian central nervous system after heavy ion radiation
NMR imaging, NMR spectroscopic, and histopathologic techniques were used to study the proton relaxation time and related biochemical changes in the central nervous system after helium beam in vivo irradiation of the rodent brain. The spectroscopic observations reported in this dissertation were made possible by development of methods for measuring the NMR parameters of the rodent brain in vivo and in vitro. The methods include (1) depth selective spectroscopy using an optimization of rf pulse energy based on a priori knowledge of N-acetyl aspartate and lipid spectra of the normal brain, (2) phase-encoded proton spectroscopy of the living rodent using a surface coil, and (3) dual aqueous and organic tissue extraction technique for spectroscopy. Radiation induced increases were observed in lipid and p-choline peaks of the proton spectrum, in vivo. Proton NMR spectroscopy measurements on brain extracts (aqueous and organic solvents) were made to observe chemical changes that could not be seen in vivo. Radiation-induced changes were observed in lactate, GABA, glutamate, and p-choline peak areas of the aqueous fraction spectra. In the organic fraction, decreases were observed in peak area ratios of the terminal-methyl peaks, the N-methyl groups of choline, and at a peak at 2.84 ppM (phosphatidyl ethanolamine and phosphatidyl serine resonances) relative to TMS. With histology and Evans blue injections, blood-brain barrier alternations were seen as early as 4 days after irradiation. 83 references, 53 figures.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6268623
- Report Number(s):
- LBL-18148; ON: DE85004509
- Resource Relation:
- Other Information: Portions are illegible in microfiche products. Thesis
- Country of Publication:
- United States
- Language:
- English
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BRAIN
BIOLOGICAL RADIATION EFFECTS
B CODES
COMPUTER CODES
D CODES
EXPERIMENTAL DATA
FOURIER TRANSFORMATION
HELIUM
HISTOLOGY
IN VIVO
ION BEAMS
N CODES
NUCLEAR MAGNETIC RESONANCE
RATS
SOLVENT EXTRACTION
SPECTROSCOPY
U CODES
ANIMALS
BEAMS
BIOLOGICAL EFFECTS
BODY
CENTRAL NERVOUS SYSTEM
DATA
ELEMENTS
EXTRACTION
FLUIDS
GASES
INFORMATION
INTEGRAL TRANSFORMATIONS
MAGNETIC RESONANCE
MAMMALS
NERVOUS SYSTEM
NONMETALS
NUMERICAL DATA
ORGANS
RADIATION EFFECTS
RARE GASES
RESONANCE
RODENTS
SEPARATION PROCESSES
TRANSFORMATIONS
VERTEBRATES
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