Phosphatidylcholine synthesis in castor bean endosperm. Metabolism of S-adenosylmethionine and ethanolamine

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6874071

Prud'homme, M P; Moore, Jr, T S ^[1]

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1. Louisiana State Univ., Baton Rouge (USA)

The methylation steps in the biosynthesis of phosphatidylcholine by castor bean endosperm have been studied. Endosperm halves were incubated with tracer concentrations of (2-{sup 14}C) ethanolamine or ({sup 14}C)S-adenosyl-L-methionine for 10 or 30 minutes, respectively. The kinetics of appearance were followed in methyl- and dimethylethanolamine, choline, and their phospho-, CDP-, and phosphatidyl-derivatives. Methyl groups from S-adenosyl-L-methionine rapidly labeled the three methylated-ethanolamine derivatives. Radioactivity then decreased in these compounds and accumulated in phosphatidylcholine. The initial methylation utilized ethanolamine as a substrate to form methyl-ethanolamine, which was partially converted to dimethyl-ethanolamine, choline, and phosphomethylethanolamine. Subsequent methylations occurred at both phospho-base and phosphatidyl-base levels. Experiments with ethanolamine confirmed these results.

• OSTI ID: 6874071
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CASTOR
CHEMICAL REACTIONS
DRUGS
ESTERS
EUPHORBIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEDICINAL PLANTS
METABOLISM
METHIONINE
METHYLATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOLIPIDS
PLANTS
SYNTHESIS
TRACER TECHNIQUES