Effect of degradative plasmid CAM-OCT on responses of Pseudomonas bacteria to UV light

Journal Article · Wed Feb 01 04:00:00 EST 1989 · J. Bacteriol.; (United States)

OSTI ID:6267204

McBeth, D L

The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT exhibited much higher UV sensitivity than did P. putida strains. UV-induced mutagenesis of plasmid-free P. putida was easily detected in three different assays (two reversion assays and one forward mutation assay), whereas UV mutagenesis of P. aeruginosa without CAM-OCT was seen only in the forward mutation assay. These results suggest major differences in DNA repair between the two species and highlight the presence of error-prone repair functions on CAM-OCT. A number of P. putida mutants carrying chromosomal mutations affecting either survival or mutagenesis after UV irradiation were isolated, and the effect of CAM-OCT on these mutants was determined. All mutations producing a UV-sensitive phenotype in P. putida were fully suppressed by the plasmid, whereas the plasmid had a more variable effect on mutagenesis mutations, suppressing some and producing no suppression of others. On the basis of the results reported here and results obtained by others with plasmids carrying UV response genes, it appears that CAM-OCT may differ either in regulation or in the number and functions of UV response genes encoded.

• Research Organization: City Univ. of New York Medical School/Sophie Davis School of Biomedical Education, City College, NY (USA)
• OSTI ID: 6267204
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 171:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
CELL CONSTITUENTS
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENETIC VARIABILITY
MICROORGANISMS
MUTATIONS
PHENOTYPE
PLASMIDS
PSEUDOMONAS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION