The CAM-OCT plasmid enhances UV responses of Pseudomonas aeruginosa recA mutants

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Journal of Bacteriology; (USA)

OSTI ID:6919889

McBeth, D L ^[1]

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1. City Univ. of New York Medical School/Sophie Davis School of Biomedical Education, City College, NY (USA)

The effect of the CAM-OCT plasmid on responses to UV irradiation of Pseudomonas aeruginosa recA mutants was characterized. Mutant alleles examined included rec-1, rec-2, and recA7::Tn501. The plasmid substantially enhanced both survival and mutagenesis of RecA- cells after treatment with UV light. Survival of the RecA-(CAM-OCT) cells after UV irradiation was intermediate between that seen in the wild-type P. aeruginosa PAO1 and the increased survival seen in PAO1(CAM-OCT) cells. Mutability was quantitated by the reversion to carbenicillin resistance of strains carrying a bla(Am) mutation on a derivative of plasmid RP1. UV-induced mutagenesis of CAM-OCT carrying recA mutants occurred at levels comparable to that seen in PAO1(CAM-OCT). The ability of CAM-OCT plasmid to suppress the recombination deficiency in recA mutants was tested by assaying for bacteriophage F116L-generalized transduction of a Tn7 insertion in the alkane utilization genes of CAM-OCT. Transduction of the Tn7 insertion was not detected in RecA-(CAM-OCT) strains but was easily seen in PAO1(CAM-OCT), indicating that the plasmid does not encode a recA analog. The results indicate that the CAM-OCT UV response genes are expressed in RecA- cells, which differs from results seen with other UV response-enhancing plasmids. The results suggest that CAM-OCT either encodes several UV responses genes itself or induces chromosomal UV response genes by an alternate mechanism.

• OSTI ID: 6919889
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:3; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE MUTATIONS
GENE RECOMBINATION
GENE REGULATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENOTYPE
MICROORGANISMS
MUTANTS
MUTATIONS
PLASMIDS
PSEUDOMONAS
RADIATION EFFECTS
RADIATION INDUCED MUTANTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SURVIVAL TIME
ULTRAVIOLET RADIATION