Effect of lindane on calcium regulation in rat brain synaptosomes

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6257232

Hawkinson, J E

It is proposed that lindane produces convulsions by enhancing Ca-dependent neurotransmitter release in the CNS. In an attempt to test this hypothesis, the effects of lindane on /sup 45/Ca fluxes in rat brain synaptosomes were examined. Under control conditions, Ca uptake by intact synaptosomes increased gradually for 19 min in low K/sup +/ medium. In high K/sup +/ medium, the synaptosomes accumulated 4.5 times as much /sup 45/Ca as in low K/sup +/ medium within 1 in. Lindane enhanced Ca uptake in low K/sup +/ medium after 1 min: the effect was maximal at 0.1 ..mu..M lindane, which increased uptake by 15.8%. However, lindane had no significant effect on Ca uptake in high K/sup +/ medium. The initial rate of Ca influx was not affected by lindane in low K/sup +/ medium. In control studies of Ca uptake by lysed synaptosomes, ATP stimulated Ca uptake by a factor of 21 after 5 min, at which time uptake was near maximal. In the presence of ATP, mitochondrial poisons reduced Ca uptake by a factor of 8 at the 5 min time point. Lindane had no effect on Ca uptake by lysed synaptosomes in the presence or absence of mitochondrial poisons and/or ATP, indicating a lack of effect on intraterminal Ca storage.

• Research Organization: California Univ., Davis (USA)
• OSTI ID: 6257232
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
BRAIN
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
INSECTICIDES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
MITOCHONDRIA
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PESTICIDES
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES