ATP-regulated neuronal catecholamine uptake: A new mechanism

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90578-F· OSTI ID:6531217

Cao, Chengjing; Eldefrawi, A T; Eldefrawi, M E ^[1]

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1. Univ. of Maryland, Baltimore (USA)

Uptake of the catecholamines (CA), dopamine (DA) and norepinephrine (NE) into synaptosomes prepared from rat and bovine brains was potentiated by ATP in a dose-dependent manner. Other nucleotides also potentiated ({sup 3}H)DA and ({sup 3}H)NE uptake. Several endogenous 5{prime}-nucleotide triphosphates potentiated ({sup 3}H)CA uptake, but were less effective than ATP. Among the ATP metabolites, only ADP potentiated uptake whereas AMP and adenosine did not. ({sup 3}H)Dopamine uptake measured in Krebs bicarbonate buffer had a km of 2.1 {mu}M and a V{sub max} of 163.9 pmol/mg prot./min. In presence of ATP, ({sup 3}H)DA uptake had much higher affinity and larger capacity than uptake in absence of added ATP. Furthermore, ({sup 3}H)DA uptake in presence of ATP had faster rate of uptake, and was independent of temperature while in absence of added ATP it was temperature-dependent. This ATP-dependent ({sup 3}H)DA uptake was retained by synaptosomal ghosts that were obtained after lysing the striatal synaptosomes and removing their contents of synaptic vesicles and mitochondria.

• OSTI ID: 6531217
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 47:7; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINES
ANIMAL CELLS
AROMATICS
ATP
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
NERVE CELLS
NEUROREGULATORS
NORADRENALINE
NUCLEOTIDES
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
REACTION KINETICS
SOMATIC CELLS
SYMPATHOMIMETICS
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE