Redox polymer-modified electrodes for the electrocatalytic regeneration of NAD

Journal Article · Tue Jun 01 00:00:00 EDT 1993 · Analytical Letters; (United States)

DOI:https://doi.org/10.1080/00032719308019888· OSTI ID:6250595

Hale, P D ^[1]; Lee, Hung-Sui ^[2]; Okamoto, Yoshi ^[3]

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1. Bio-Tek Instruments, In., Winooski, VT (United States)
2. Brookhaven National Lab., Upton, NY (United States)
3. Polytechnic Univ., Brooklyn, NY (United States)

A new siloxane polymer containing covalently attached Meldola Blue has been synthesized and shown to mediate the oxidation of the reduced nicotinamide adenine dinucleotide cofactor (NADH). Cyclic voltammetry and stationary potential experiments demonstrate that spectrographic graphite electrodes coated with this polymer can electrocatalytically regenerate NAD at a substantially lower overvoltage (e.g., O m V vs. SCE) than can unmodified electrodes. These polymer-modified electrodes should be useful as transducers in dehydrogenase-based biosensors.

• OSTI ID: 6250595
• Journal Information: Analytical Letters; (United States), Journal Name: Analytical Letters; (United States) Vol. 26:6; ISSN ANALBP; ISSN 0003-2719
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400400* -- Electrochemistry
CATALYSTS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
COENZYMES
DESIGN
ELECTROCATALYSTS
ELECTRODES
IONS
NAD
NUCLEOTIDES
ORGANIC COMPOUNDS
OVERVOLTAGE
OXIDATION
REDOX PROCESS
REGENERATION
REPROCESSING
SEPARATION PROCESSES
SYNTHESIS
TRANSDUCERS
VOLTAMETRY