Investigation of the Ionization Mechanism of NAD{sup +}/NADH-Modified Gold Electrodes in ToF-SIMS Analysis

Journal Article · Wed Aug 15 04:00:00 EDT 2018 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/S13361-018-1983-1· OSTI ID:22776868

Hua, Xin; Zhao, Li-Jun; Long, Yi-Tao ^[1]

---

1. East China University of Science and Technology, Key Laboratory for Advanced Materials and Department of Chemistry (China)

Analysis of nicotinamide adenine dinucleotide (NAD{sup +}/NADH)-modified electrodes is important for in vitro monitoring of key biological processes. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to analyze NAD{sup +}/NADH-modified gold electrodes. Interestingly, no obvious characteristic peaks of nicotinamide fragment could be observed in the mass spectra of NAD{sup +}/NADH in their neutral sodium pyrophosphate form. However, after acidification, the characteristic peaks for both NAD{sup +} and NADH were detected. This was due to the suppression effect of inner pyrophosphoric salts in both neutral molecules. Besides, it was proved that the suppression by inner salt was intramolecular. No obvious suppression was found between neighboring molecules. These results demonstrated the suppression effect of inner salts in ToF-SIMS analysis, providing useful evidence for the study of ToF-SIMS ionization mechanism of organic molecule-modified electrodes. .

• OSTI ID: 22776868
• Journal Information: Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry Journal Issue: 8 Vol. 29; ISSN 1044-0305; ISSN JAMSEF
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
GOLD
ION MICROPROBE ANALYSIS
IONIZATION
MASS SPECTRA
MASS SPECTROSCOPY
NICOTINAMIDE
PHOSPHATES
SODIUM
TIME-OF-FLIGHT METHOD