A reagentless enzymatic amperometric biosensor using vertically aligned carbon nanofibers (VACNF)

Journal Article · Mon Dec 31 23:00:00 EST 2007 · Sensors and Actuators B: Chemical

DOI:https://doi.org/10.1016/j.snb.2008.01.060· OSTI ID:1055198

Weeks, Martha L ^[1]; Rahman, Touhidur ^[2]; Frymier, Paul Dexter ^[2]; Islam, Syed K ^[1]; McKnight, Timothy E ^[2]

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1. University of Tennessee, Knoxville (UTK)
2. ORNL

A reagentless amperometric enzymatic biosensor is constructed on a carbon substrate for detection of ethanol. Yeast alcohol dehydrogenase (YADH), an oxidoreductase, and its cofactor nicotinamide adenine dinucleotide (NAD+) are immobilized by adsorption and covalent attachment to the carbon substrate. Carbon nanofibers grown by plasma enhanced chemical vapor deposition (PECVD) are chosen as the electrode material due to their excellent structural and electrical properties. Electrochemical techniques are employed to test the functionality and performance of the biosensor using reduced form of nicotinamide adenine dinucleotide (NADH) which also determines the oxidation peak potential of NADH. Subsequently, amperometric measurements are conducted for detection of ethanol to determine the electrical current response due to the increase in analyte concentration. The detection range, storage stability, reusability, and response time of the biosensor are also examined.

• Research Organization: Oak Ridge National Laboratory (ORNL); Center for Nanophase Materials Sciences
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1055198
• Journal Information: Sensors and Actuators B: Chemical, Journal Name: Sensors and Actuators B: Chemical Journal Issue: 1 Vol. 133; ISSN 0925-4005
• Country of Publication: United States
• Language: English