Determination of the radiation sensitivity of the stromal cells in the murine long-term bone marrow culture by measuring the induction and rejoining of interphase chromosome breaks

Journal Article · Fri Apr 02 04:00:00 EST 1993 · International Journal of Radiation Oncology, Biology and Physics; (United States)

DOI:https://doi.org/10.1016/0360-3016(93)90312-J· OSTI ID:6238611

Kodym, R ^[1]; Hoerth, E ^[2]

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1. Univ. of Ulm (Germany)
2. Univ. of Vienna (Austria)

The purpose of this work was to determine the radiosensitivity of bone marrow stromal cells, the rate of interphase chromosome breakage and rejoining of stromal cells in the murine long term bone marrow culture and of human skin fibroblasts were compared. The cells were irradiated with doses up to 6 Gy and repair times up to 6 hr were investigated. After induction of premature chromosome condensation by fusing the cells with mitotic HeLa cells, the number of interphase chromosome fragments was counted. The number of radiation induced breaks was found to be not significantly different for both cell types with 6.16 [+-] 0.26 breaks per Gray for the fibroblasts and 5.96 [+-] 0.20 breaks per Gray for the stromal cells. A significant difference was observed in the repair rate. The fibroblasts rejoined 39.6% of the breaks induced initially during the first hour after irradiation and 5.6 [+-] 1.84 breaks remained unrejoined after 6 hr, while the stromal cells were able to rejoin 63.2% in 1 hr and had 2.05 [+-] 0.07 breaks unrejoined after 6 hr. If the well substantiated assumption is made, that the capacity to repair DNA double strand breaks or interphase chromosome breaks is correlated with the cellular radiosensitivity, these findings indicate that murine bone marrow stromal cells are more radioresistant than human skin fibroblasts. 30 refs., 2 figs.

• OSTI ID: 6238611
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (United States), Journal Name: International Journal of Radiation Oncology, Biology and Physics; (United States) Vol. 25:5; ISSN IOBPD3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DNA REPAIR
FIBROBLASTS
MUTATIONS
RADIOSENSITIVITY
REPAIR
SOMATIC CELLS
STRAND BREAKS