Rejoining of prematurely condensed chromosomes in radiosensitive xrs-5 cells
Conference
·
OSTI ID:400752
- Thomas Jefferson Univ., Philadelphia, PA (United States). Dept. of Radiation Oncology
Xrs-5 cells, a radiosensitive, DNA double strand break repair deficient mutant of CHO cells have been studied with the premature chromosome condensation (PCC) technique. This mutant displayed a higher number of initial chromosome breaks with x-ray treatment as well as partial deficiency in the rejoining of interphase chromosome breaks with the standard PCC protocol. Moreover, hypertonic treatment during the incubation period which allowed for PCC did not change the yield of PCC breaks in x-irradiated xrs-5 cells. Notably the number of PCC breaks after treatment with hypertonic media is similar in CHO and xrs-5 cells. Recently, a gene product responsible for the xrs phenotype was identified as a Ku-like DNA end binding protein. The present paper summarizes completed information regarding the induction and repair of the {alpha}- and {beta}-forms of PCC breaks in xrs-5 cells and demonstrates that this gene product predominantly affects the fast form ({beta}-form) of interphase chromosome breaks.
- OSTI ID:
- 400752
- Report Number(s):
- CONF-9410280--; ISBN 0-935470-90-5
- Country of Publication:
- United States
- Language:
- English
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