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(Analysis of DNA damage and mutations induced by radon daughter products): Progress report

Technical Report ·
OSTI ID:6571180
The goal of the project is to better understand the genetic and somatic risks associated associated with radon exposure by characterizing both the DNA lesions induced by radon and the mechanisms by which both these lesions are processed. In order to accomplish this goal, we are exposing mammalian cells in vitro to the radon daughter product /sup 212/Bi and analyzing DNA damage at the cellular, cytogenetic and molecular level. In addition, we are examining the influence of DNA repair systems on /sup 212/Bi-induced cytotoxicity, DNA/chromosome breakage and gene mutations. The experiments to date have involved treatment of four different Chinese hamster ovary (CHO) cell lines with /sup 212/Bi and x rays to measure: (1) DNA and chromosome break induction, (2) cell killing, and (3) mutation induction at the HGPRT locus. The four CHO cell lines include AA8, EM9, CHO-K1 and XRS-5. They differ in radiosensitivity and in their ability to rejoin DNA single- and double-strand breaks. EM9 is deficient in its ability to rejoin DNA single-strand breaks and XRS-5 is deficient in its ability to rejoin DNA double-strand breaks. 2 figs., 2 tabs.
Research Organization:
Chicago Univ., IL (USA)
DOE Contract Number:
FG02-88ER60661
OSTI ID:
6571180
Report Number(s):
DOE/ER/60661-1; ON: DE89004860
Country of Publication:
United States
Language:
English

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Related Subjects

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
320107* -- Energy Conservation
Consumption
& Utilization-- Building Systems-- (1987-)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALPHA DECAY RADIOISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BISMUTH 212
BISMUTH ISOTOPES
CELL CULTURES
CELL KILLING
DAUGHTER PRODUCTS
DAYS LIVING RADIOISOTOPES
DNA REPAIR
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ENZYME INDUCTION
EVEN-EVEN NUCLEI
GENE MUTATIONS
GENE REGULATION
HAMSTERS
HEAVY NUCLEI
HOURS LIVING RADIOISOTOPES
IN VITRO
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
PROGRESS REPORT
RADIATIONS
RADIOISOTOPES
RADON 222
RADON ISOTOPES
RECOVERY
REPAIR
RODENTS
SOMATIC MUTATIONS
STRAND BREAKS
VERTEBRATES
X RADIATION