Liquid chromatography-mass spectrometry with electron impact and fast ion bombardment with a ribbon storage interface

Journal Article · Tue Sep 01 04:00:00 EDT 1981 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00234a016· OSTI ID:6232638

Smith, R D; Burger, J E; Johnson, A L

The design and performance of a new moving ribbon liquid chromatograph-mass spectrometer interface for use with secondary ion mass spectrometry (SIMS) and conventional electron impact ionizaton (EI) are described and initial results are presented. The interface includes a 120 cm long region at atmospheric pressure and aerosol deposition of the LC effluent to allow the complete evaporation of the LC mobile phase before the first vacuum slit and a 320 cm total ribbon length to allow the storage of chromatographically separated materials. Detection limits for several amino several amino acids of < 10/sup -11/ g have been demonstrated. The long ribbon also allows the temporary storage of 5 to 60 min HPLC separations on the ribbon for subsequent reanalysis by SIMS or EI (at several temperatures) and has been demonstrated for amino acids using SIMS and complex samples using electron impact. Surface damage using SIMS due to high primary ion currents was found to be limited by the short exposure time under typical conditions. A new method for ribbon cleaning using vapor deposition of a thin layer of silver is also described which reduces background from contaminants and residue on the ribbon and is superior to heaters or solvent baths for ribbon cleaning. The approach to the data acquisition, potential artifacts related to surface damage, and initial attempts at quantitation are described.

• Research Organization: Pacific Northwest Lab., Richland, WA
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6232638
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 53:11; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
400105 -- Separation Procedures
AMINO ACIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DESIGN
EQUIPMENT
INTERFACES
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROMETERS
MASS SPECTROSCOPY
MEASURING INSTRUMENTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PERFORMANCE
SEPARATION PROCESSES
SPECTROMETERS
SPECTROSCOPY