Effect of puromycin aminonucleoside on HSPG core protein content of glomerular epithelial cells

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6230137

Kasinath, B S; Singh, A K; Kanwar, Y S; Lewis, E J ^[1]

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1. Northwestern Univ. Medical School, Chicago, IL (USA)

It has been suggested that the glomerular basement membrane heparan sulfate proteoglycan (HSPG) is an important determinant of the glomerular permselectivity barrier. Derangements in the content of basement membrane heparan sulfate have been implicated in alterations in glomerular permselectivity seen in many glomerular diseases such as aminonucleoside nephrosis. The cellular origin and metabolism of the glomerular basement membrane HSPG have not been studied in detail. The authors have detected the expression of the proteoglycan by cloned glomerular visceral epithelial cells of the rat by employing a specific antibody against the core protein of HSPG isolated from the rat glomerular basement membrane. These findings suggest that in the rat in vivo glomerular visceral epithelial cells are one source of heparan sulfate present in the glomerular basement membrane. The effect of puromycin aminonucleoside (PAN) on the HSPG core protein content of the cloned glomerular epithelial cells was studied. By a quantitative immunoperoxidase method, the aminonucleoside caused a 28% reduction in the core protein content of the epithelial cells following 72 h of incubation. However, the content of Heymann nephritis-related antigen, Fx1A was unchanged. Studies employing ({sup 3}H)leucine incorporation showed that PAN was a weak inhibitor of de novo protein synthesis at 24 h of incubation, with complete recovery at 48 and 72 h. These data suggest that PAN effect on heparan sulfate core protein cannot be attributed to generalized inhibition of protein synthesis. The precise mechanism underlying the aminonucleoside effect on heparan sulfate core protein remains to be elucidated.

• OSTI ID: 6230137
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:4; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL TISSUES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMISTRY
DRUGS
EPITHELIUM
FLUORESCENCE
GLOMERULI
GLUCOPROTEINS
GLYCOPROTEINS
HYDROGEN COMPOUNDS
KIDNEYS
LEUCINE
LUMINESCENCE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
PROTEINS
PUROMYCIN
SACCHARIDES
TISSUES
TRITIUM COMPOUNDS
UPTAKE