Unaltered anionic sites of glomerular basement membrane in aminonucleoside nephrosis

Journal Article · Sun Apr 01 00:00:00 EST 1984 · Kidney Int.; (United States)

DOI:https://doi.org/10.1038/ki.1984.65· OSTI ID:6136142

Kanwar, Y S; Jakubowski, M L

Quantitative electron microscopic autoradiography was employed to determine the changes in the binding of /sup 125/I-cationic ferritin (CFI, pI approximately 7.2 to 7.4) to the anionic sites of the glomerular basement membrane (GBM) of rats following the induction of nephrosis. Animals were rendered nephrotic by a single intravenous injection of puromycin aminonucleoside (PAN) and sacrificed 0, 7, 14, and 21 days after its administration. CFI (10 mg/mCi/100 g body weight) was given intravenously. The kidneys were subsequently fixed by perfusion and processed for electron microscopic autoradiography. The mean grain densities over the GBMs on 0, 7, 14, and 21 days of PAN nephrosis were 1.50 +/- 0.04, 1.49 +/- 0.05, 1.50 +/- 0.05, and 1.51 +/- 0.04, respectively. These results indicate that there are no significant alterations in the anionic sites rich in heparan sulfate proteoglycan during the entire course of PAN nephrosis.

• Research Organization: Northwestern Univ. Medical School, Chicago, IL
• OSTI ID: 6136142
• Journal Information: Kidney Int.; (United States), Vol. 25:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
KIDNEYS
AUTORADIOGRAPHY
NEPHRITIS
PATHOGENESIS
CELL MEMBRANES
CHEMICAL BONDS
IODINE 125
LABELLED COMPOUNDS
PUROMYCIN
RATS
TRACER TECHNIQUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
UROGENITAL SYSTEM DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques