Characterization of a novel heparan sulfate proteoglycan found in the extracellular matrix of liver sinusoids and basement membranes

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Journal of Cell Biology; (United States)

DOI:https://doi.org/10.1083/jcb.113.5.1231· OSTI ID:5595869

Soroka, C J; Farquhar, M G ^[1]

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1. Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut (USA)

A novel heparan sulfate proteoglycan (HSPG) present in the extracellular matrix of rat liver has been partially characterized. Proteoglycans were purified from a high salt extract of total microsomes from rat liver and found to consist predominantly ({approximately} 90%) of HSPG. A polyclonal antiserum raised against this fraction specifically recognized HSPG by immunoprecipitation and immunoblotting. The intact, fully glycosylated HSPG migrated as a broad smear (150-300 kD) by SDS-PAGE, but after deglycosylation with trifluoromethanesulfonic acid only a single {approximately} 40-kD band was seen. By immunocytochemistry this HSPG was localized in the perisinusoidal space of Disse associated with irregular clumps of basement membrane-like extracellular matrix material, some of which was closely associated with the hepatocyte sinusoidal cell surface. It was also localized in biosynthetic compartments (rough ER and Golgi cisternae) of hepatocytes, suggesting that this HSPG is synthesized and deposited in the space of Disse by the hepatocyte. The anti-liver HSPG IgG also stained basement membranes of hepatic blood vessels and bile ducts as well as those of kidney and several other organs (heart, pancreas, and intestine). An antibody that recognizes the basement membrane HSPG found in the rat glomerular basement membrane did not precipitate the 150-300-kD rat liver HSPG. We conclude that the liver sinusoidal space of Disse contains a novel population of HSPG that differs in its overall size, its distribution and in the size of its core protein from other HSPG (i.e., membrane-intercalated HSPG) previously described in rat liver. It also differs in its core protein size from HSPG purified from other extracellular matrix sources. This population of HSPG appears to be a member of the basement membrane HSPG family.

• OSTI ID: 5595869
• Journal Information: Journal of Cell Biology; (United States), Journal Name: Journal of Cell Biology; (United States) Vol. 113:5; ISSN 0021-9525; ISSN JCLBA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOASSAY
BIOLOGICAL LOCALIZATION
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ELECTROPHORESIS
EXTRACELLULAR SPACE
FRACTIONATION
GLANDS
GLYCOPROTEINS
IMMUNOASSAY
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LIVER
MAMMALS
MEMBRANES
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
RATS
RODENTS
SEPARATION PROCESSES
SPACE
SULFATES
SULFUR COMPOUNDS
TRACER TECHNIQUES
VERTEBRATES