Pyrimidine nucleoside, pseudouridine, and modified nucleoside excretion by growing and resting fibroblasts. [Hamster embryos]
We are examining the relationship of RNA metabolism and de novo pyrimidine synthesis as parameters of malignant transformation. These initial experiments on normal hamster embryo fibroblasts have shown that excreted nucleosides are markers for intracellular RNA metabolism. We employed affinity chromatography to concentrate the nucleosides in the medium and sensitive column chromatographic procedures to quantitatively measure them. The excretion of pyrimidine nucleoside from hamster embryo fibroblasts in culture was found to be dependent on the growth stage of the cells, with the greatest accumulation occurring during cell quiescence. The major nucleosidee excretion products, uridine and cytidine, were both normal end products of RNA metabolism and the major nucleoside excretion products from cultured cells. The modified nucleosides N-1-methylguanosine, N-2-methylguanosine, N-2-dimethylguanosine, N-4-acetylcytidine, N-1-methylinosine, pseudouridine, N-1-methyladenosine, N-3-methylcytidine, and 5-methylcytidine were found, as were several unidentified nucleosides.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 6228535
- Journal Information:
- J. Cell. Physiol.; (United States), Vol. 99:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NUCLEOSIDES
EXCRETION
PYRIMIDINES
BIOSYNTHESIS
RNA
METABOLISM
CHROMATOGRAPHY
EMBRYOS
EXPERIMENTAL DATA
FIBROBLASTS
GRAPHS
HAMSTERS
ANIMAL CELLS
ANIMALS
AZINES
CLEARANCE
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
HETEROCYCLIC COMPOUNDS
INFORMATION
MAMMALS
NUCLEIC ACIDS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RIBOSIDES
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550200* - Biochemistry