Composition and characterization of tRNA from Methanococcus vannielii

Journal Article · Sun Jan 01 04:00:00 EST 1978 · J. Bacteriol.; (United States)

OSTI ID:7287105

Best, A N

Purified bulk tRNA from Methanococcus vanniellii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N/sup 2/-methylguanosine, N/sup 2/,N/sup 2/-dimethylguanosine, thiolated nucleosides pseudouridine, dihydrouridine, and O/sup 2/'-methylcytidine were quantitated. In vitro methylation by M. vannielii extracts with S-adenosylmethionine and undermethylated E. coli tRNA revealed active tRNA methyltransferases for formation of methylated residues found in native M. vannielii tRNA, but none for the formation of 7-methylguanosine or ribothymidine. The native M. vannielii tRNA became methylated in the 7-methylguanosine position by E. coli extracts, but ribothymidine was not formed. Both M. vannielii and E. coli tRNA methyltransferases produced unidentified methylated residues in tRNA's lacking or deficient in ribothymidine.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7287105
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 133:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BIOCHEMISTRY
CHEMICAL COMPOSITION
CHEMISTRY
CHROMATOGRAPHY
CRYOGENIC FLUIDS
ENZYMES
FLUIDS
HYDROCARBONS
METHANE
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
RIBOSIDES
RNA
SEPARATION PROCESSES
TRANSFERASES