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RNA turnover in cultured hamster embryo cells: identification of modified nucleoside end products

Journal Article · · J. Cell. Physiol.; (United States)
 [1];
  1. Univ. of Tennessee-Oak Ridge Graduate School of Biomedical Sciences
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Fourteen methylated nucleosides (N-2-dimethylGuo, N-2-methylGuo, N-1-methylGuo, N-5-methylUrd, N-3-methylUrd, N-1-methylAdo, N-3-methylCyd, N-5-methylCyd, N-1-methylIno, 2'-0-methyl-Cyd, 2'-0-methylUrd, 2'-0-methylGuo, 2'-0-methylino, and thymidine) and one methylated base (m/sup 7/Gua) have been identified as normal excretion products of cultured hamster embryo cells. The methylated nucleosides are excreted in the culture media subsequent to RNA turnover. The excretion pattern of the base-methylated nucleosides was determined by continuous labeling of serum-stimulated quiescent hamster embryo cells with (/sup 3/H-CH/sub 3/)methionine and measurement of radioactivity in the excreted nucleosides between 23 and 81 1/2 hours after the label was added. These nucleosides accumulate exponentially until a maximum level is reached after 60 hours. These maximum levels were maintained for at least an additional 20 hours.

DOE Contract Number:
W-7405-ENG-26
OSTI ID:
6124029
Journal Information:
J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 114:1; ISSN JCLLA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL CULTURES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DRUGS
EMBRYONIC CELLS
HAMSTERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LABELLING
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
METHYLATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
REACTION KINETICS
RIBOSIDES
RNA
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES