Cell kinetics in mouse lung following administration of carcinogens and butylated hydroxytoluene

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(85)90254-6· OSTI ID:6228412

Witschi, H P; Morse, C C

A series of experiments is described which was designed to test the hypothesis that, in mouse lung, enhancement of tumor development could occur independently of overall alveolar cell hyperplasia. Male A/J mice were given 1000 mg/kg of urethane or 10 mg/kg of 3-methylcholanthrene (MCA). Alveolar cells were labeled through continuous infusion of (TH)thymidine for 6 weeks after administration of the carcinogen. Urethane produced a significant hyperplasia of the type II alveolar cell population, whereas MCA had no such effect. Five repeated injections of 300 mg/kg of butylated hydroxytoluene (BHT), a procedure known to enhance lung tumor development, produced cell hyperplasia only during the first 2 weeks; later the mice became resistant to the action of BHT. In animals treated with piperonyl butoxide prior to BHT, cell proliferation was abolished. BHT still had a small but significant enhancing effect on tumor development. However, this effect was dwarfed by the observation that piperonyl butoxide alone greatly inhibited tumor development. The data do not allow exclusion of alveolar cell hyperplasia as a mechanism in BHT-mediated enhancement of mouse lung tumor development. 19 references, 4 figures, 3 tables.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6228412
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 78; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
550901 -- Pathology-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATED AROMATICS
ANIMALS
AROMATICS
AZINES
BIOCHEMICAL REACTION KINETICS
BODY
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARCINOGENS
CELL PROLIFERATION
DATA
DISEASES
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LUNGS
MAMMALS
MICE
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIMIDINES
REACTION KINETICS
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
THYMIDINE
TOLUENE
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URETHANE
VERTEBRATES