The effect of inhibition of hexose-monophosphate shunt on the metabolism of glucose and function in rat brain in vivo
Rats treated 4 hr previously with 6-aminonicotinamide showed a twenty-four fold increase of (/sup 14/C)phosphogluconate in the adult brain at 30 min after intection of (U-/sup 14/C)glucose indicating a blockade of the hexosemmonophosphate shunt. There was a significant increase in the /sup 14/C-content of glucose and glucose-6-phosphate, and a decrease in that of amino acids. (/sup 14/C)Phosphoglycerate content showed no consistent change after 6-aminonicotinamide treatment. The concentration of glucose and glucose 6-phosphate increased significantly without a significant change in the lactate pool in the brain of 6-aminonicotinamide treated rats. The rate of utilization of glucose in the brain of control rats was 0.73 mumol/min per g of brain. It decreased by 16% in rats treated with 6-aminonicotinamide; the results suggested that both glycolysis and pyruvate oxidation were affected. The amount of glucose utilized in the brain by the hexosemonophosphate shunt was approximately 0.0093 mumol/min per g of brain, i.e. 1.3% of the total rate of utilization of glucose. The observed changes were not due to hypothermia. The rate of glucose utilization was higher in animals exposed to higher ambient temperature and to stress caused by handling. The results were explained by postulating a role for the hexosemonophosphate shunt in providing neurotransmitter amino acids glutamate and gamma-aminobutyrate, and interdependence of brain function and glucose utilization.
- Research Organization:
- Department of Biochemistry, St. George's Hospital Medical School, London
- OSTI ID:
- 6225200
- Journal Information:
- Neurochem. Res.; (United States), Vol. 7:9
- Country of Publication:
- United States
- Language:
- English
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