Automated fluorometric method for hydrogen peroxide in air

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00294a024· OSTI ID:6207877

Lazrus, A L; Kok, G L; Lind, J A; Gitlin, S N; Heikes, B G; Shetter, R E

A fluorometric method for measuring H/sub 2/O/sub 2/ vapor in air utilizes peroxidase enzyme to catalyze the reaction in which hydroperoxides cause dimerization of (p-hydroxyphenyl)acetic acid. In a second channel, H/sub 2/O/sub 2/ is selectively decomposed by catalase so that the fluorescence signal is due only to organic hydroperoxides. The difference between the two signals is a measure of H/sub 2/O/sub 2/ vapor. The H/sub 2/O/sub 2/ vapor is collected by means of a glass coil though which air and water flow concurrently. The coefficient of variation is 0.5% at 2.5 parts per billion by volume. The standard deviation of the base line is 10 parts per trillion by volume (pptv) under laboratory conditions. This standard deviation has varied between 3 and 33 pptv during ground-based field missions, and was 70 pptv on aircraft flights. Thirty seconds is required for the signal to change from 10 to 90% of its maximum value. 13 references, 4 figures, 1 table.

• Research Organization: National Center for Atmospheric Research, Boulder, CO
• OSTI ID: 6207877
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 58:3; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
ACETIC ACID
AIR
AIR SAMPLERS
AUTOMATION
CARBOXYLIC ACIDS
CATALYTIC EFFECTS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DATA
DECOMPOSITION
DIMERIZATION
EMISSION SPECTROSCOPY
ENZYMES
EQUIPMENT
EXPERIMENTAL DATA
FLUIDS
FLUORESCENCE SPECTROSCOPY
GASES
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
INFORMATION
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PERFORMANCE
PEROXIDASES
PEROXIDES
POLYMERIZATION
QUANTITATIVE CHEMICAL ANALYSIS
SAMPLERS
SAMPLING
SPECTROSCOPY
TRACE AMOUNTS