Fluorometric determination of hydrogen peroxide in groundwater

Journal Article · Sun Feb 15 04:00:00 EST 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00131a010· OSTI ID:6814210

Holm, T R; George, G K; Barcelona, M J

The fluorometric scopoletin-horseradish peroxidase method has been modified for field determinations of hydrogen peroxide concentrations in groundwaters. Standard additions calibration compensates for background fluorescence and inconsistent stoichiometry of the fluorescence quenching reaction due to interferences by the matrix. The detection limit, defined as the blank plus three standard deviations, ranged from 3.6 to 44.6 nM. However, this limit was more an indication of the difficulty of preparing peroxide-free water than the actual limit imposed by the sensitivity of the method for the peroxide contamination introduced with the reagents. For 111 field determinations the weighted average (uncorrected) hydrogen peroxide concentration was 20.2 nM and the pooled standard deviation was 7.7 nM. The average of 45 field blanks was 7.8 nM with a pooled standard deviation of 5.2 nM. At nanomolar concentration levels, it is essential that samples are analyzed for H/sub 2/O/sub 2/ in the field. Storage periods exceeding 1 h caused serious errors and irreproducible results.

• Research Organization: Illinois Dept. of Energy and Natural Resources, Champaign
• OSTI ID: 6814210
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:4; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
DATA
EMISSION SPECTROSCOPY
ENZYMES
EXPERIMENTAL DATA
FLUORESCENCE SPECTROSCOPY
GROUND WATER
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
INFORMATION
NUMERICAL DATA
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PEROXIDES
QUANTITATIVE CHEMICAL ANALYSIS
REDOX POTENTIAL
REDOX REACTIONS
SPECTROSCOPY
TRACE AMOUNTS
WATER
WATER CHEMISTRY