Hydrogen peroxide metabolism in soybean embryonic axes at the onset of germination. [Glycine max]

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.86.2.626· OSTI ID:7151735

Puntarulo, S; Sanchez, R A; Boveris, A ^[1]

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1. Universidad de Buenos Aires (Argentina)

Hydrogen peroxide steady state levels of 5 micromolar were determined in soybean (Glycine max) embryonic axes incubated for 2 hours and in axes pretreated with aminotriazole or cyanide, where these levels were 50 and 1 micromolar, respectively. The activities of catalase (105 picomoles H{sub 2}O{sub 2} per minute per axis), peroxidase (10-44 picomoles H{sub 2}O{sub 2} per minute per axis), glutathione peroxidase (3 picomoles H{sub 2}O{sub 2} per minute per axis) and superoxide dismutase (3.5 units per axis), were also determined. Catalase seems to be the most important H{sub 2}O{sub 2} consuming enzyme at the physiological concentration of H{sub 2}O{sub 2} level. A short treatment with aminotriazole, while substantially increasing H{sub 2}O{sub 2} level, did not affect the growth of the axes. The production of superoxide anion by the mitochondria isolated from soybean axes was measured from the superoxide dismutase-sensitive rate of adrenochrome formation in the presence of NADH or succinate as substrate and amounted to 1.3 and 0.8 nanomole O{sub 2}{sup {minus}} per minute per milligram protein, respectively. According to the stoichiometry of O{sub 2}{sup {minus}} and H{sub 2}O{sub 2} dismutation reactions, it is apparent that about 0.9 to 1.5% of the total oxygen uptake proceeds through the formation of the free intermediates of the partial reduction of oxygen.

• OSTI ID: 7151735
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 86:2; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ANIONS
AZOLES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CATALASE
CELL CONSTITUENTS
CHARGED PARTICLES
COENZYMES
CYANIDES
DICARBOXYLIC ACIDS
DRUGS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GERMINATION
GLUTATHIONE
GLYCINE HISPIDA
GROWTH
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
IONS
KINETICS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METABOLISM
MITOCHONDRIA
NADH2
NONMETALS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
OXYGEN
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDASES
PEROXIDES
PLANT GROWTH
PLANTS
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
SEEDS
SUCCINIC ACID
SUPEROXIDE DISMUTASE
TRIAZOLES
UPTAKE