Potential mediation of somatostatin secretion from canine fundic D-cells by protein kinase c

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6207869

Chiba, T; Sugano, K; Park, J; Yamada, T

The authors examined the possible importance of protein kinase c-dependent mechanisms in mediating the stimulatory effects of gastrin and cholecystokinin (CCK) on the release of somatostatin-like immunoreactivity (SLI) from isolated canine fundic D-cells. Diacylglycerides, presumably the products of phosphoinositide breakdown that activate protein kinase c, and phospholipase C, which catalyzes the production of endogenous diacylglycerides from membrane phospholipids, both stimulated SLI secretion in a dose-dependent fashion. Both classes of agents potentiated the actions of adenosine 3', 5'-cyclic monophosphate-dependent agonists but not those of gastrin and CCK. The stimulatory effects of gastrin and CCK correlated with their abilities to enhance the incorporation of /sup 32/P into membrane phosphatidyl inositol and phosphatidic acid and promote the release of (/sup 3/H)inositol trisphosphate from prelabeled D-cells, two parameters of phosphoinositide turnover. These data suggest that protein kinase c may serve to transduce the signals activated by gastrin and CCK in D-cells.

• Research Organization: Univ. of Michigan Medical Center, Ann Arbor (USA)
• OSTI ID: 6207869
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARBOHYDRATES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOGS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
GASTRIN
GASTROINTESTINAL TRACT
HORMONES
HYDROLASES
INOSITOLS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KININS
LABELLED COMPOUNDS
LEUCINE
LIGHT NUCLEI
LIPASE
MAMMALS
MEMBRANE PROTEINS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SACCHARIDES
SECRETION
SOMATOSTATIN
STOMACH
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES