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Carbamoylcholine and gastrin induce inositol lipid turnover in canine gastric parietal cells

Journal Article · · American Journal of Physiology; (USA)
OSTI ID:6834467
; ; ; ; ;  [1]
  1. Univ. of Michigan Medical School, Ann Arbor (USA)
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The potential role of inositol phospholipid turnover in mediating acid secretion was examined in a preparation enriched for isolated canine gastric parietal cells. The stimulatory effects of carbamoylcholine (carbachol) and gastrin on parietal cell uptake of ({sup 14}C)aminopyrine were linked to dose- and time-dependent selective reduction in cellular phosphatidylinositol content, although the specific fatty acid composition of the phosphoinositides was not altered. Analysis of ({sup 3}H)inositol phosphates accumulated in cells prelabeled with ({sup 3}H)inositol revealed an increase in labeled inositol trisphosphate by 5 min of incubation with either carbachol or gastrin. Furthermore, after preincubation of parietal cells in medium containing ({sup 32}P)orthophosphate, the two secretagogues elicited a time-dependent decrease in {sup 32}P labeling of phosphatidylinositol 4,5-bisphosphate and concomitant increase in labeling of phosphatidic acid. These data demonstrate that the acid secretagogue actions of carbachol and gastrin are correlated with turnover of cellular inositol phospholipids in a preparation consisting predominantly of parietal cells.
OSTI ID:
6834467
Journal Information:
American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:1; ISSN 0002-9513; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOGS
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
ESTERS
GASTRIN
GASTROINTESTINAL TRACT
HORMONES
HYDROGEN COMPOUNDS
INOSITOL
INOSITOLS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SECRETION
STOMACH
TIME DEPENDENCE
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES