Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: Identification of 11q23 translocation breakpoints with a yeast artificial chromosome

Journal Article · Fri Nov 30 23:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.23.9358· OSTI ID:6203112

Rowley, J D; Diaz, M O; Espinosa, R III; Patel, Y D; van Melle, E; Ziemin, S; Le Beau, M M ^[1]; Taillon-Miller, P; Domer, P H ^[2]; Lichter, P; Ward, D C ^[3]; Evans, G A ^[4]; Kersey, J H ^[5]

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1. Univ. of Chicago, IL (United States)
2. Washington Univ. School of Medicine, St. Louis, MO (United States)
3. Yale Univ. School of Medicine, New Haven, CT (United States)
4. Salk Institute, La Jolla, CA (United States)
5. Univ. of Minnesota, Minneapolis (United States)

Translocations involving chromosome 11, band q23, are frequent recurring abnormalities in human acute lymphoblastic and acute myeloid leukemia. The authors used 19 biotin labeled probes derived from genes and anonymous cosmids for hybridization to metaphase chromosomes from leukemia cells that contained four translocations involving band 11q23: t(4;11)(q21;q23), t(6;11)(q27;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13). The location of the cosmid probes relative to the breakpoint in 11q23 was the same in all translocation. Of the cosmid clones containing known genes, CD3D was proximal and PBGD, THY1, SRPR, and ETS1 were distal to the breakpoint on 11q23. Hybridization of genomic DNA from a yeast clone containing yeast artificial chromosomes (YACs), that carry 320 kilobases (kb) of human DNA including CD3D and CD3G genes, showed that the YACs were split in all four translocations. These results indicate that the breakpoint at 11q23 in each of these translocations occurs within the 320 kb encompassed by these YACs; whether the breakpoint within the YACs is precisely the same in the different translocation is presently unknown.

• DOE Contract Number: FG02-86ER60408
• OSTI ID: 6203112
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:23; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AZOLES
BIOTIN
CARBOXYLIC ACIDS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DISEASES
DNA HYBRIDIZATION
EUMYCOTA
FLUORESCENCE
FUNGI
GENETIC MAPPING
HETEROCHROMOSOMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
IMIDAZOLES
IMMUNE SYSTEM DISEASES
LEUKEMIA
LUMINESCENCE
MAPPING
MICROORGANISMS
MOLECULAR BIOLOGY
MUTATIONS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATIENTS
PLANTS
VITAMIN B GROUP
VITAMINS
YEASTS