Localization of neurotensin binding sites in rat kidney

Journal Article · · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/0196-9781(82)90012-2· OSTI ID:6192872

Quirion, R; Gaudreau, P; St-Pierre, S; Rioux, F

(/sup 3/H)Neurotensin ((/sup 3/H)NT) appears to bind specifically to a single class of sites in slide-mounted rat kidney sections (KD . 8.3 nM; Bmax . 31.6 fmol/mg tissue). Bound (/sup 3/H)NT can be displaced by nonradioactive NT and a series of its fragments and analogues with relative potencies that correlate well (r . 0.91; p less than 0.005) to their potencies in the rat stomach strip bioassay. These results suggest that NT receptors are similar in both systems. However, they are probably slightly different from those present in the guinea pig atria (r . 0.78; p less than 0.1). Researchers visualized these sites by using the tritium-sensitive LKB film technique analysed by computerized densitometry. (/sup 3/H)NT binding sites are highly concentrated in the renal cortex while low levels are observed in the renal medulla. The possible physiological and/or pathophysiological significances of the presence of (/sup 3/H)NT binding sites in the kidney are discussed.

• Research Organization: Neurosciences Branch, National Institute of Mental Health, Bethesda, MD
• OSTI ID: 6192872
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Journal Name: Peptides (Fayetteville, N.Y.); (United States) Vol. 3:5; ISSN PPTDD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BIOASSAY
BIOLOGICAL LOCALIZATION
BODY
HORMONES
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
NORTH AMERICA
ORGANS
PHOTOGRAPHIC FILMS
QUANTITY RATIO
RATS
RECEPTORS
RESEARCH PROGRAMS
RODENTS
TRITIUM COMPOUNDS
USA
VERTEBRATES