Characterization and visualization of cholecystokinin receptors in rat brain using (/sup 3/H)pentagastrin

Journal Article · · Peptides (Fayetteville, N.Y.); (United States)

OSTI ID:6223427

Gaudreau, P; Quirion, R; Pierre, S; Pert, C B

(/sup 3/H)Pentagastrin binds specifically to an apparent single class of CCK receptors on slide-mounted sections of rat brain (KD . 5.6 nM; Bmax . 36.6 fmol/mg protein). This specific binding is temperature-dependent and regulated by ions and nucleotides. The relative potencies of C-terminal fragments of CCK-8(SO/sup 3/H), benzotript and proglumide in inhibiting specific (/sup 3/H)pentagastrin binding to CCK brain receptors reinforce the concept of different brain and pancreas CCK receptors. CCK receptors were visualized by using tritium-sensitive LKB film analyzed by computerized densitometry. CCK receptors are highly concentrated in the cortex, dentate gyrus, granular and external plexiform layers of the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, claustrum, accumbens nucleus, some nuclei of the amygdala, thalamus and hypothalamus.

• Research Organization: National Inst. of Mental Health, Bethesda, MD
• OSTI ID: 6223427
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Vol. 4:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
BIOCHEMICAL REACTION KINETICS
GASTRIN
RECEPTORS
DENSITOMETERS
PHOTOMETRY
RATS
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CENTRAL NERVOUS SYSTEM
HORMONES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEASURING INSTRUMENTS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PHOTOMETERS
POLYPEPTIDES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques