Autoradiographic distribution of (/sup 3/H)neurotensin receptors in rat brain: visualization by tritium-sensitive film

Journal Article · · Peptides (Fayetteville, N.Y.); (United States)

OSTI ID:5999090

Quirion, R; Gaudreau, P; St-Pierre, S; Rioux, F; Pert, C B

(/sup 3/H)Neurotensin ((/sup 3/H)NT) binds specifically to a single class of binding sites on slides-mounted sections of rat brain 1Kp . 5.1 nM; Bmax . 16.2 fmol/mg tissue). Bound (/sup 3/H)NT can be displaced by nonradioactive NT and a series of its fragments and analogues with relative potencies that correlate closely (r . 0.89; p less than 0.01) to their potencies in the rat stomach strip bioassay. These results suggest that NT receptors are similar in both systems. (/sup 3/H)NT binding sites were visualized by using tritium-sensitive LKB film analysed by computerized densitometry. (/sup 3/H)NT receptors are highly concentrated in the external layer of the olfactory bulb, in the rhinal sulcus, in certain nuclei of the amygdala, in the substantia nigra, zona compacta and in the ventral tegmental area. The high density of (/sup 3/H)NT receptors in the last two areas suggest an interaction between NT and brain dopaminergic systems such as the nigrostriatal and the mesolimbic pathways.

• Research Organization: Neurosciences Branch, National Institute of Mental Health, Bethesda, MD
• OSTI ID: 5999090
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Journal Name: Peptides (Fayetteville, N.Y.); (United States) Vol. 3:5; ISSN PPTDD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
HORMONES
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
NERVE CELLS
NERVOUS SYSTEM
NORTH AMERICA
ORGANS
PHOTOGRAPHIC FILMS
RATS
REACTION KINETICS
RECEPTORS
RESEARCH PROGRAMS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
USA
VERTEBRATES