Mechanisms of immunological eradication of a syngeneic guinea pig tumor. II. Effect of methotrexate treatment and T cell depletion of the recipient on adoptive immunity
The influence of methotrexate on the development of immunity to the line 10 hepatoma was studied in guinea pigs. Chronic methotrexate treatment had no apparent effect on the ability of immune guinea pigs to suppress the growth of inoculated tumor cells. In contrast, the same methotrexate regimen inhibited the development of tumor immunity if started before the 8th day after immunization with a vaccine containing viable line 10 cells admixed with Bacillus Calmette-Guerin (BCG) cell walls. Thus, methotrexate selectively inhibited the afferent limb of the immune response. In adoptive transfer experiments, methotrexate-treated recipient guinea pigs were capable of being passively sensitized with immune spleen cells, indicating that the primary cell-mediated immune response of the recipient was not required for adoptive immunity. The contribution of recipient T cells in adoptive immunity was further investigated in guinea pigs deleted of T cells by thymectomy, irradiation, and bone marrow reconstitution. Despite demonstrable deficiency in T lymphocyte reactions, B animals were fully capable of rejecting tumors after transfer of immune cells. These results suggest that the expression of adoptive immunity was independent of recipient T cell participation. In addition, sublethal irradiation of immune spleen cells prior to adoptive transfer abolished their efficacy. Proliferation of transferred immune cells in the recipient may be essential for expression of adoptive immunity.
- Research Organization:
- Laboratory of Immunobiology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland
- OSTI ID:
- 6185123
- Journal Information:
- Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 35:1; ISSN TRPLA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Suppression of adoptive antituberculosis immunity by normal recipient animals
Protection against Mycobacterium tuberculosis infection by adoptive immunotherapy. Requirement for T cell-deficient recipients
Related Subjects
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMOTHERAPY
CONNECTIVE TISSUE CELLS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
GUINEA PIGS
HEPATOMAS
IMMUNE REACTIONS
IMMUNITY
IMMUNOTHERAPY
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
METHOTREXATE
NEOPLASMS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SUBLETHAL IRRADIATION
THERAPY
TUMOR CELLS
VACCINES
VERTEBRATES