Structural comparison and chromosomal localization of the human and mouse IL-13 genes

Journal Article · Tue Jun 15 04:00:00 EDT 1993 · Journal of Immunology; (United States)

OSTI ID:6180320

McKenzie, A N.J.; Sato, A; Doyle, E L; Zurawski, G ^[1]; Li, X; Milatovich, A; Francke, U ^[2]; Largaespada, D A; Copeland, N G; Jenkins, N A ^[3]

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1. DNAX Research Institute of Cellular and Molecular Biology, Palo Alto, CA (United States)
2. Stanford Univ. Medical School, CA (United States)
3. National Cancer Institute, Frederick, MD (United States)

The genomic structure of the recently described cytokine IL-13 has been determined for both human and mouse genes. The nucleotide sequence of a 4.6-kb DNA segment of the human gene is described. The human IL-13 gene (IL 13) occurs as a single copy in the haploid genome and maps to human chromosome 5. A 4.3-kb DNA fragment of the mouse IL-13 gene (Il 13) has been sequenced and found to occur as a single copy, mapping to mouse chromosome 11. Intrachromosomal mapping studies revealed that both genes contain four exons and three introns and show a high degree of sequence identify throughout their length. Potential recognition sequences for transcription factors that are present in the 5'-flanking region and are conserved between both genes include IFN-responsive elements, binding sites for AP-1, AP-2, and AP-3, an NF-lL 6 site, and a TATA-like sequence. Both genes map to chromosomal locations adjacent to genes encoding other cytokines, including IL-3, GM-CSF, IL-5, and IL-4 suggesting that IL-13 is another member of this cytokine gene family that may have arisen by gene duplication. 26 refs., 5 figs., 3 tabs.

• OSTI ID: 6180320
• Journal Information: Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 150:12; ISSN JOIMA3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CHROMOSOMES
DNA SEQUENCING
GENE AMPLIFICATION
GENES
GENETIC MAPPING
GROWTH FACTORS
HUMAN CHROMOSOME 5
HUMAN CHROMOSOMES
LYMPHOKINES
MAMMALS
MAN
MAPPING
MICE
MITOGENS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS
VERTEBRATES