An intron capture strategy used to identify and map a lysyl oxidase-like gene on chromosome 9 in the mouse

Journal Article · Sat Mar 01 04:00:00 EST 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1996.4574· OSTI ID:518482

Wydner, K S; Passmore, H C ^[1]; Kim, Houngho; Csiszar, K; Boyd, C D ^[2]

---

1. Rutgers Univ., Piscataway, NJ (United States)
2. UMDNJ, New Brunswick, NJ (United States)

An intron capture strategy involving use of polymerase chain reaction was used to identify and map the mouse homologue of a human lysyl oxidase-like gene (LOXL). Oligonucleotides complementary to conserved domains within exons 4 and 5 of the human lysyl oxidase-like gene were used to amplify the corresponding segment from mouse genomic DNA. Sequencing of the resulting mouse DNA fragment of approximately 1 kb revealed that the exon sequences at the ends of the amplified fragment are highly homologous (90% nucleotide identity) to exons 4 and 5 of the human lysyl oxidase-like gene. An AluI restriction site polymorphism within intron 4 was used to map the mouse lysyl oxidase-like gene (Loxl) to mouse Chromosome 9 in a region that shares linkage conservation with human chromosome 15q24, to which the LOXL was recently mapped. 22 refs., 3 figs.

• OSTI ID: 518482
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 40; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMES
DNA SEQUENCING
EXONS
GENE MUTATIONS
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 15
INTRONS
MICE
OLIGONUCLEOTIDES
OXIDOREDUCTASES
POLYMERASE CHAIN REACTION
RFLPS
STRUCTURE-ACTIVITY RELATIONSHIPS