Activation of RNA polymerase III transcription of human Alu repetitive elements by adenovirus type 5: Requirement for the E1b 58-Kilodalton protein and the products of E4 open reading frames 3 and 6

Journal Article · Tue Jun 01 00:00:00 EDT 1993 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.13.6.3231· OSTI ID:6179650

Panning, B; Smiley, J R ^[1]

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1. McMaster Univ., Ontario (Canada)

Alu elements are the single most abundant class of dispersed repeated sequences in the human genome, comprising 5-10% of the mass of human DNA. This report demonstrates that Ad5 infection strongly stimulates Pol III transcription of human Alu elements in HeLa and 293 cells. In contrast to the cases of Ad5-induced Pol III transcriptional activation, this process requires the E1b 58-kDa protein and the products of E4 open reading frames (ORFs) 3 and 6 in addition to the E1a 289-residue product. These findings suggest novel regulatory properties of the Ad5 E1b and E4 proteins and raise the possibility that analogous cellular trans-acting factors serve to modulate Alu expression in vivo.

• OSTI ID: 6179650
• Journal Information: Molecular and Cellular Biology; (United States), Vol. 13:6; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
GENETIC EFFECTS
RNA POLYMERASES
TRANSCRIPTION
DNA
DNA SEQUENCING
GENETIC MAPPING
GENETICS
IN VIVO
MASS
PROTEINS
RIBOSOMES
RNA
SUBCELLULAR DISTRIBUTION
BIOLOGICAL EFFECTS
BIOLOGY
CELL CONSTITUENTS
DISTRIBUTION
ENZYMES
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VIRUSES
550400* - Genetics
550300 - Cytology