Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Activation of the human beta interferon gene by the adenovirus type 12 E1B gene

Journal Article · · J. Virol.; (United States)
OSTI ID:5432855
;
The transcription of endogenous beta interferon mRNA was activated in human embryo kidney (HEK) cells infected with adenovirus 12 (Ad12) but was activated only inefficiently or not at all in HEK cells infected with Ad5 and rc-1 (Ad5 dl312 containing the Ad12 E1A region). The analysis with Ad12 mutants showed that Ad12 E1B products, especially the 19K protein, were important for the expression of the endogenous beta interferon gene and Ad12 E1A products were not involved in the expression. The expression of exogeneously transfected pIFN-CAT (a hybrid plasmid having the human beta interferon promoter fused with the CAT gene) was activated in HEK and chicken embryo fibroblast (CEF) cells infected with either Ad12 or Ad5. The analysis of cotransfection of CEF cells with pIFN-CAT and plasmids containing fragments of Ad12 or Ad5 DNA showed that Ad12 or Ad5 E1B (possibly the 19K protein) was and E1A was not involved in the expression of the exogenous pIFN-CAT.
Research Organization:
Univ. of Tokyo, Japan
OSTI ID:
5432855
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:1; ISSN JOVIA
Country of Publication:
United States
Language:
English

Similar Records

Tumorigenicity and adenovirus-transformed cells: Collagen interaction and cell surface laminin are controlled by the serotype origin of the E1A and E1B genes
Journal Article · Sun Jan 31 23:00:00 EST 1988 · Journal of Virology; (USA) · OSTI ID:6522831
Inhibition of interferon-inducible gene expression by adenovirus E1A proteins: Block in transcriptional complex formation
Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:5604232
Transcription of interferon-stimulated genes is induced by adenovirus particles but is suppressed by E1A gene products
Journal Article · Thu Dec 31 23:00:00 EST 1987 · J. Virol.; (United States) · OSTI ID:5438195

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOCHEMISTRY
BIRDS
BODY
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CHEMISTRY
CHICKENS
CHLORAMPHENICOL
CONNECTIVE TISSUE CELLS
DNA
DRUGS
EMBRYONIC CELLS
ENZYMES
FIBROBLASTS
FOWL
GENE REGULATION
GENES
GROWTH FACTORS
INTERFERON
KIDNEYS
LABELLED COMPOUNDS
LYMPHOKINES
MAMMALS
MAN
MESSENGER-RNA
MICROORGANISMS
MITOGENS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PLASMIDS
PRIMATES
PROTEINS
RNA
SOMATIC CELLS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES
VIRUSES