Incorporation of a subacute test with zebra fish into a hierarchical system for evaluating the effect of toxicants in the aquatic environment

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Ecotoxicology and Environmental Safety; (USA)

DOI:https://doi.org/10.1016/0147-6513(90)90048-A· OSTI ID:6173390

Neilson, A H; Allard, A S; Fischer, S; Malmberg, M; Viktor, T ^[1]

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1. Swedish Environmental Research Institute, Stockholm (Sweden)

Single-species laboratory tests were used to assess the acute toxicity of halogenated phenolic compounds. No single test system was most sensitive to all of the compounds examined, substantial variations in the sensitivity of the various organisms were noted, and there was no correlation between the toxicities assayed with different test systems. The zebra fish (Brachydanio rerio) embryo/larvae system was used to examine subacute effects using two of the compounds, and a protocol was developed with 6 weeks preexposure to the toxicant. Preexposure decreased the lowest observable effect concentration by a factor of about 4, and the effect was completely reversible during a 6-week postexposure period in the absence of the toxicant. An enclosed system for carrying out the zebra fish embryo/larvae test was developed and evaluated with three neutral volatile compounds: the median survival time and the frequency of occurrence of deformation were examined as end points. The effect of pH on toxicity was evaluated in buffered media for four of the test systems: toxicity increased markedly at the lower pH values, and it could be shown that the ionized forms of the phenols were not the only contributors to toxicity. It is proposed that the zebra fish system incorporating preexposure could be incorporated into a hierarchical system using a range of organisms for assessing acute toxicity in single species under laboratory conditions and multicomponent systems simulating natural ecosystems.

• OSTI ID: 6173390
• Journal Information: Ecotoxicology and Environmental Safety; (USA), Journal Name: Ecotoxicology and Environmental Safety; (USA) Vol. 20:1; ISSN EESAD; ISSN 0147-6513
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL INDICATORS
EMBRYOS
FISHES
LARVAE
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
POLLUTION
SENSITIVITY
SURVIVAL TIME
TOXICITY
VERTEBRATES
WATER POLLUTION