Repair of experimentally produced defects in rabbit articular cartilage by autologous chondrocyte transplantation
Using the knee joints of New Zealand White rabbits, a baseline study was made to determine the intrinsic capability of cartilage for healing defects that do not fracture the subchondral plate. A second experiment examined the effect of autologous chondrocytes grown in vitro on the healing rate of these defects. To determine whether any of the reconstituted cartilage resulted from the chondrocyte graft, a third experiment was conducted involving grafts with chondrocytes that had been labeled prior to grafting with a nuclear tracer. Results were evaluated using both qualitative and quantitative light microscopy. Macroscopic results from grafted specimens displayed a marked decrease in synovitis and other degenerative changes. In defects that had received transplants, a significant amount of cartilage was reconstituted (82%) compared to ungrafted controls (18%). Autoradiography on reconstituted cartilage showed that there were labeled cells incorporated into the repair matrix.
- Research Organization:
- Hospital for Joint Diseases Orthopaedic Institute, New York, NY (USA)
- OSTI ID:
- 6172096
- Journal Information:
- J. Orthop. Res.; (United States), Journal Name: J. Orthop. Res.; (United States) Vol. 7:2; ISSN JORED
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of donor age on DNA repair by articular chondrocytes
Ultrastructure of chondrocytes of articular cartilage soon after irradiation
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
BONE JOINTS
CARTILAGE
CONNECTIVE TISSUE
HEALING
HETEROCYCLIC COMPOUNDS
INJURIES
LABELLED COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RABBITS
RECOVERY
REPAIR
RIBOSIDES
SKELETON
THYMIDINE
TISSUES
TRANSPLANTS
TRITIUM COMPOUNDS
VERTEBRATES
WOUNDS