The repair of full-thickness articular cartilage defects. Immune responses to reparative tissue formed by allogeneic growth plate chondrocyte implants
- Department of Orthopaedics, Shimane Medical School, Izumo (Japan)
Growth plate cartilage cultivated in vitro was attached with a fibrin clot to a full-thickness articular cartilage defect on knee joints in allogeneic New Zealand rabbits. The healing of the defects was assessed by gross examination, light microscopy, and immunologic analysis for 24 weeks. Immunologic assessment of cell-mediated immunity, cytotoxicity of a humoral antibody by a 51 chromium release assay, and immunofluorescence studies were carried out. During the first two weeks following grafting, healing was excellent in 11 of the 17 defects. From three to 24 weeks, 11 of 42 defects examined had good results. Host lymphocytes had accumulated around the allograft at two to 12 weeks. Most of the implanted cartilage grown in vitro died and was replaced by fibrous tissue. The immunologic studies suggested that the implanted cartilage began to degenerate two to three weeks after implantation partially because of a humoral immune response but more importantly because of cell-mediated cytotoxicity.
- OSTI ID:
- 5390914
- Journal Information:
- Clinical Orthopaedics and Related Research; (United States), Journal Name: Clinical Orthopaedics and Related Research; (United States) Vol. 268; ISSN 0009-921X; ISSN CORTB
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY
BODY AREAS
BODY FLUIDS
BONE JOINTS
CARTILAGE
CHROMIUM 51
CHROMIUM ISOTOPES
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CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FIBRIN
FLUORESCENCE
GRAFTS
HEALING
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNE REACTIONS
INJURIES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEGS
LEUKOCYTES
LIMBS
LUMINESCENCE
LYMPHOCYTES
MAMMALS
MATERIALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
RADIOISOTOPES
RECOVERY
REPAIR
SCLEROPROTEINS
SKELETON
SOMATIC CELLS
TISSUE CULTURES
TISSUES
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES
WOUNDS