Stereospecific inositol 1,4,5-( sup 32 P)trisphosphate binding to isolated rat liver nuclei: Evidence for inositol trisphosphate receptor-mediated calcium release from the nucleus

Journal Article · Fri Nov 30 23:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.23.9270· OSTI ID:6171295

Malviya, A N; Rogue, P; Vincendon, G ^[1]

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1. Centre de Neurochimie, Centre National de la Recherche Scientifique, Strasbourg (France)

It is well known that inositol 1,4,5-trisphosphate binding and release of calcium are mediated by the same protein. Several reports have indicated the location of the inositol 1,4,5-trisphosphate receptor in organelles other than endoplasmic reticulum. Immunocytochemical studies on the subcellular localization of 1,4,5-trisphosphate receptor in the Purkinje cells from two laboratories have given contradictory results regarding the nuclear location of this receptor. In this paper, a high-affinity inositol 1,4,5-({sup 32}P)trisphosphate binding site (Kd = 0.11 nM) on nuclei isolated from rat liver and devoid of any microsomal, mitochondrial, or plasma membrane constituents is documented. Furthermore, we present data demonstrating that inositol 1,4,5-trisphosphate is capable of releasing {sup 45}Ca{sup 2+} from the intact isolated liver nuclei. A rapid and transient release of calcium that was taken up by nuclei in the presence of ATP is observed. The role of inositol 1,4,5-trisphosphate in the coupling between cytoplasmic second messengers and nuclear events activated during signal transduction is postulated.

• OSTI ID: 6171295
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:23; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
CELL NUCLEI
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ESTERS
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
LIVER
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MICROSOMES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RIBOSOMES
RODENTS
SECRETION
TRACER TECHNIQUES
VERTEBRATES