Ethanol stimulates calcium mobilization, phosphoinositide turnover and shape change in human platelets

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6168624

Rubin, R; Hoek, J B

The effect of ethanol of cytosolic free calcium levels was investigated in human platelets which were loaded with the intracellular calcium indicator FURA-2. Administration of ethanol in the concentration range of 50-300 mM resulted in a transient, dose-dependent increase in cytosolic calcium, maximal within 5 seconds and returning to near basal levels over the next 5 minutes. Parallel incubations of platelets with the same concentrations of ethanol stimulated shape change as detected in an aggregometer. Chelation of external calcium with EGTA prior to the addition of ethanol reduced the calcium burst partially, but not completely, whereas shape change was largely unaffected. Addition of ethanol to /sup 32/P-labeled platelets resulted in a 16% decrease in the level of /sup 32/P-phosphatidylinositol 4,5-bisphosphate and a 14% increase in /sup 32/P-phosphatidylinositol 4-phosphate within 2 minutes. /sup 32/P-phosphatidic acid levels increased rapidly within 30 seconds and rose linearly thereafter. Phosphatidylinositol and phosphatidylcholine were unaffected by ethanol. The results indicate that ethanol mobilizes intracellular calcium by activation of phosphoinositide-specific phospholipase C, thereby stimulating platelet shape change.

• Research Organization: Thomas Jefferson Univ. School of Medicine, Philadelphia, PA
• OSTI ID: 6168624
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CALCIUM
CARBOXYLIC ACIDS
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
EGTA
ELEMENTS
ESTERS
ETHANOL
GLYCOLS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
MAN
MATERIALS
MEMBRANE TRANSPORT
METALS
MORPHOLOGICAL CHANGES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
SECRETION
TRACER TECHNIQUES
VERTEBRATES