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Ethanol stimulates phosphoinositide-specific phospholipase C in rat hepatocytes

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5090237
; ;
Ethanol (50-300 mM) causes a transient mobilization of calcium from hormone-sensitive pools in rat hepatocytes. They have studied the action of ethanol on polyphosphoinositide and inositol phosphate levels in these cells. Addition of ethanol to isolated hepatocytes prelabelled with /sup 32/Pi resulted in a small (3-5%) decrease in the level of (/sup 32/P)-phosphatidylinositol 4,5-bisphosphate and a 10-15% increase in (/sup 32/P) phosphatidylinositol 4-phosphate and (/sup 32/P) phosphatidic acid. Maximal changes were seen within 30 sec after ethanol. (/sup 32/P) phosphatidylcholine was unaffected. The changes were concentration dependent over a range of 50-500 mM ethanol. Phorbol esters, which inhibit ethanol-induced calcium mobilization, also inhibited these effects of ethanol on polyphosphoinositides. In intact hepatocytes labeled with myo-(2-/sup 3/H)inositol a significant ethanol-induced increase in (/sup 3/H)inositol phosphates could not be detected. After permeabilization of the cells with digitonin, however, ethanol caused a time and concentration dependent release of (/sup 3/H) inositol trisphosphate and (/sup 3/H)inositol bisphosphate. These results indicate that ethanol-induced calcium mobilization in hepatocytes is due to the activation by ethanol of the phosphoinositide-specific phospholipase C.
Research Organization:
Hahnemann Univ. School of Medicine, Philadelphia, PA
OSTI ID:
5090237
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBOXYLESTERASES
CARCINOGENS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
ETHANOL
HYDROLASES
HYDROXY COMPOUNDS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPASE
LIPIDS
LIVER CELLS
MAMMALS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHORBOL ESTERS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES