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GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
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Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared from (/sup 32/P)labelled platelets. In the presence of guanosine 5'-(3-O)-thiotriphosphate (GTP..gamma..S), thrombin increased the release of inositol triphosphate and inositol biphosphate approximately 500%. GTP..gamma..S alone stimulated release 2 fold. Maximal activation of thrombin-induced phosphoinositide hydrolysis was observed at 10 ..mu..M GTP. Although addition of calcium had no effect, 2 mM EGTA completely inhibited inositolphosphate release. Addition of high speed supernatant to (/sup 32/P)labelled membranes stimulated the release of inositolphosphates. This hydrolysis was further enhanced by the addition of GTP. These data demonstrate that the breakdown of polyphosphoinositides in isolated platelet membranes is dependent on GTP and stimulated by platelet cytosol.

Research Organization:
American Red Cross Bi-State Chapter, St. Louis, MO
OSTI ID:
7171498
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:2; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CALCIUM COMPOUNDS
CARBOHYDRATES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHELATING AGENTS
CHEMICAL REACTIONS
COAGULANTS
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DRUGS
EGTA
ENZYMES
ESTERASES
ESTERS
EXPERIMENTAL DATA
GLYCOLS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
INFORMATION
INHIBITION
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPASE
LIPIDS
LYSIS
MATERIALS
MEMBRANES
MONOSACCHARIDES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
SACCHARIDES
SERINE PROTEINASES
SOLVOLYSIS
STIMULATION
THROMBIN
TRACER TECHNIQUES