Potentiometric analysis of the cytochromes of an Escherichia coli mutant strain lacking the cytochrome d terminal oxidase complex
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6167380
A combination of potentiometric analysis and electrochemically poised low-temperature difference spectroscopy was used to examine a mutant strain of Escherichia coli that was previously shown by immunological criteria to be lacking the cytochrome d terminal oxidase. It was shown that this strain is missing cytochromes d, a/sub 1/, and b/sub 558/ and that the cytochrome composition of the mutant is similar to that of the wild-type strain grown under conditions of high aeration. The data indicate that the high-aeration branch of the respiratory chain contains two cytochrome components, b/sub 556/ (midpoint potential (E/sub m/) = + 35 mV) and cytochrome o (E/sub m/ = + 165 mV). The latter component binds to CO and apparently has a reduced-minus-oxidized split-..cap alpha.. band with peaks at 555 and 562 mn. When the wild-type strain was g
- Research Organization:
- Univ. of Illinois, Urbana
- DOE Contract Number:
- AC02-80ER10682
- OSTI ID:
- 6167380
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 157:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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