Immunological investigation of the distribution of cytochromes related to the two terminal oxidases of Escherichia coli in other gram-negative bacteria

Journal Article · Fri Feb 01 04:00:00 EST 1985 · J. Bacteriol.; (United States)

OSTI ID:5666316

Kranz, R G; Gennis, R B

Monospecific antibodies were raised against the two terminal oxidase complexes of the aerobic respiratory chain of Escherichia coli. These are the cytochrome d and cytochrome o complexes. The antibodies were used to check for the occurrence of cross-reactive antigens in membrane preparations from a variety of gram-negative bacteria by rocket immunoelectrophoresis and immunoblotting techniques. With these criteria, proteins closely related to the cytochrome d complex of E. coli appeared to be widely distributed. Among the strains containing cytochrome d-related material were Serratia marcescens, Photobacterium phosphoreum, Salmonella typhimurium, Klebsiella pneumoniae, and Azotobacter vinelandii. The data suggest that the d-type terminal oxidase in many of these strains is associated in a complex with b-type and a/sub 1/-type cytochromes, as has been found to be the case in E. coli. K. pneumoniae and S. typhimurium were also shown to have material cross-reactive to the E. coli cytochrome o complex.

• Research Organization: Univ. of Illinois, Urbana
• DOE Contract Number: AC02-80ER10682
• OSTI ID: 5666316
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 161:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
AZOTOBACTER
BACTERIA
BIOASSAY
CELL CONSTITUENTS
CELL MEMBRANES
CYTOCHROME OXIDASE
CYTOCHROMES
ENZYME IMMUNOASSAY
ENZYMES
ESCHERICHIA COLI
HAEM DEHYDROGENASES
IMMUNITY
KLEBSIELLA
MEMBRANES
MICROORGANISMS
OXIDOREDUCTASES
PHOTOSYNTHETIC BACTERIA
PIGMENTS
RESPIRATION
SALMONELLA
SALMONELLA TYPHIMURIUM
SERRATIA